Correction of uneven illumination (vignetting) in digital microscopy images

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Correction of uneven illumination (vignetting) in digital microscopy images.

BACKGROUND Many digital microscopy images suffer from poor illumination at the peripheries (vignetting), often attributable to factors related to the light path between the camera and the microscope. A commonly used method for correcting this has been to use the illumination of an empty field as a correction filter (white shading correction). AIMS To develop an alternative shading correction ...

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The presence of systematic noise in images in high-throughput microscopy experiments can significantly impact the accuracy of downstream results. Among the most common sources of systematic noise is non-homogeneous illumination across the image field. This often adds an unacceptable level of noise, obscures true quantitative differences and precludes biological experiments that rely on accurate...

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ژورنال

عنوان ژورنال: Journal of Clinical Pathology

سال: 2003

ISSN: 0021-9746

DOI: 10.1136/jcp.56.8.619